From former site, http://www.gsmnp.com/pages/fishing.html
Had this old info posted:

"In addition, the following streams and their tributaries upstream from the points described are closed to fishing, so that the native brook trout (brookie) can be protected. For exact location, consult the appropriate USGS 1:24,000 Quadrangle Map available at all Park visitor centers.
North Carolina
1. Gunter Creek at the first trail crossing on Gunter at 3240' elevation.
2. Big Creek and Yellow Creek at their junction.
3. McGinty Creek at its confluence with Swallow Fork.
4. Correll Branch at the junction with Little Cataloochee Creek.
5. Lost Bottom Creek at its confluence with Palmer Creek at 3280' elevation.
6. Bunches Creek at the Park boundary.
7. Stillwell Creek at the Park boundary.
8. Straight Fork and Balsam Corner Creek at their common junction.
9. Raven Fork at Big Pool which is the confluence of Left Fork, Middle Fork and Right Fork (also known as Three Forks).
10. Enloe Creek at the junction with Raven Fork.
11. Taywa Creek at its confluence with Bradley Fork.
12. Chasm Prong and Gulf Prong at their common junction on Bradley Fork.
13. Sahlee Creek at its confluence with Deep Creek.
14. Noland Creek and Salola Branch at their confluence.
15. Huggins Creek (tributary of Forney Creek) at the cascade at 3700' elevation.
16. Hazel Creek at the cascades.
17. Walkers Creek at the falls at 3400' elevation.
18. Defeat Branch at its junction with Bone Valley Creek.
19. Gunna Creek (tributary to Eagle Creek) at trail crossing at 3080' elevation.

Tennessee
1. Sams Creek at the confluence with Thunderhead Prong.
2. Marks Creek at the falls at 2600'.
3. Lynn Camp Prong at campsite #28 (Mark's Cove).
4. Indian Flats Prong at the Middle Prong trail crossing.
5. Meigs Creek at its confluence with Little River.
6. Fish Camp Prong and Goshen Creek at their common junction.
7. Little River and Grouse Creek at their common junction.
8. Road Pr on at its confluence with West Prong of Little Pigeon River.
9. Buck Fork and Middle Prong of the Little Pigeon River at their common junction.
10. Dunn Creek at Park boundary.
11. Indian Cam Creek at Park boundary.
12. Greenbrier River (Little Creek) at Park boundary.
13. Toms Creek at its junction with Cosby Creek.
14. Cosby Creek where Low Gap Trail crosses the stream.
15. Rock Creek at its junction with Cosby Creek .
16. Spruce Flats Creek at its confluence with Middle Prong of Little River.
17. Meigs Post Prong at its confluence with Little River."

In checking current regs, please note that some stream sections remain off-limits, namely upstream of the point described below, including feeder waters:

North Carolina
Bear Creek at its junction with Forney Creek.
Tennessee
Sams Creek at the confluence with Thunderhead Prong
Indian Flats Prong at the Middle Prong Trial crossing